Methods and apparatus for sequential amplification reactions
US12123052B2 · kind B2 · utility
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59References
20Claims
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Key dates
| Filing date | Dec 22, 2020 |
| Grant date | Oct 22, 2024 |
| Priority date | — |
| Expiry date | Aug 4, 2042 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0644
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.