Heavy peptide approach to accurately measure unprocessed C-terminal lysine
US12123879B2 · kind B2 · utility
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12Claims
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Key dates
| Filing date | Jun 18, 2021 |
| Grant date | Oct 22, 2024 |
| Priority date | — |
| Expiry date | Jan 11, 2043 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6854
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present disclosure provides a method for accurately measuring post-translational modifications in proteins such as antibodies. In particular, the method pertains to the use of heavy isotopic standards to generate a calibration curve to allow for accurate quantitation of a modified peptide. The method may be used to accurately quantify C-terminal truncation in antibodies using mass spectrometry.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.