Patent · US Active

Construction method and applications of glycosyltransferase BS-YjiC mutant

US12134789B2 · kind B2 · utility

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Assignee

North-West University · ZA

Inventors

Weina Li · South Glastonbury, US
Daidi Fan · Xi'an, CN
Chenhui Zhu · Tuji, CN

Key dates

Filing date	Mar 25, 2024
Grant date	Nov 5, 2024
Priority date	—
Expiry date	Mar 25, 2044

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y204/01
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A glycosyltransferase BS-YjiC mutant, a construction method and an application thereof provided, relating to the field of genetic engineering technologies. The glycosyltransferase BS-YjiC mutant is obtained by performing site-directed mutation on amino acids at the 125th, 178th, 313th, 125th and 178th, or 125th and 313th positions of a wild-type glycosyltransferase BS-YjiC with the amino acid sequence as shown in SEQ ID NO: 1. Compared with the wild-type glycosyltransferase BS-YjiC, the glycosyltransferase BS-YjiC mutant is more suitable for catalyzing protopanaxadiol (PPD) to generate rare ginsenosides F12 and Rh2, which is more conducive to the flexibility of the production process.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.