Patent · US Active

Biotin-streptavidin cleavage composition and library fragment cleavage

US12146133B2 · kind B2 · utility

0Cited by

0References

11Claims

0Family size

Assignee

Illumina, Inc. · US

Inventors

Dan Cao · Beijing, CN
Jeffrey S. Fisher · San Francisco, US
Fiona Kaper · San Diego, US
Tarun Khurana · San Diego, US
Tong Liu · Longbeilingcun, CN
Burak Okumus · San Diego, US
Victor J. Quijano · Mountain View, US
Clifford Lee Wang · Redwood City, US
Yir-Shyuan Wu · Fremont, US
Shi Min Xiao · Hong Kong, CN
Hongxia Xu · Shandong, CN

Key dates

Filing date	Feb 3, 2021
Grant date	Nov 19, 2024
Priority date	—
Expiry date	Mar 13, 2043

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6806
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

An example of a biotin-streptavidin cleavage composition includes a formamide reagent and a salt buffer. The formamide reagent is present in the biotin-streptavidin cleavage composition in an amount ranging from about 10% to about 50%, based on a total volume of the biotin-streptavidin cleavage composition. The salt buffer makes up the balance of the biotin-streptavidin cleavage composition. In some examples, the biotin-streptavidin cleavage composition is used to cleave library fragments from a solid support. In other examples, other mechanisms are used to cleave library fragments from a solid support.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.