Methods for promoting entry of an agent into a cell

US12150951B2 · kind B2 · utility

Assignee

• TECREA LIMITED · GB

Inventors

• Liam Good · London, GB
• Kantaraja Chindera · London, GB
• Valentina Gburcik · London, GB

Key dates

• Filing date: Jan 11, 2019
• Grant date: Nov 26, 2024
• Expiry date: Mar 31, 2041

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B82Y5/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A method for promoting entry of an agent (introduced agent) into a cell, the method comprising the step of complexing the introduced agent in the presence of an entry-promoting agent and then exposing to cells, wherein the entry-promoting agent comprises a linear and/or branched or cyclic polymonoguanide/polyguanidine, polybiguanide, analogue or derivative thereof according to the following Formula 1a & b. The method also provides a means for formation of nanoparticles formed between the entry promoting agent and the introduced agent. wherein: “n”, refers to number of repeating units in the polymer, and n can vary from 2 to 1000, for example from 2 or 5 to 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, 200, 250, 300, 350, 400, 450, 500, 600, 700, 800 or 900; G1 and G2 independently represent a cationic group comprising biguanide or guanidine, wherein L1, and L2 are directly joined to a Nitrogen atom of the guanide; L1 and L2 are linking groups between the G1 and G2 cationic groups in the polymer and independently represent an aliphatic group containing C1-C40 carbon atoms, for example an alkyl group such as methylene, ethylene, propylene, C4, C5, C6, C7, C8, C9 or C10; C1-C10, -C20,…