Permanent epigenetic gene silencing

US12152240B2 · kind B2 · utility

Assignees

• OSPEDALE SAN RAFFAELE S.R.L. · IT
• FONDAZIONE TELETHON · IT

Inventors

• Luigi Naldini · Torino, IT
• Angelo Leone Lombardo · Milano, IT
• Angelo Amabile · Milano, IT
• Alessandro Migliara · Milano, IT

Key dates

• Filing date: Oct 23, 2015
• Grant date: Nov 26, 2024
• Expiry date: Oct 23, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2800/80
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A product comprising two or more artificial transcription repressors (ATRs), or polynucleotides encoding therefor, selected from groups (a), (b), (c) or (d): (a) an ATR comprising a DNA-binding domain operably linked to a KRAB domain or homologue thereof; (b) an ATR comprising a DNA-binding domain operably linked to a DNMT3A, DNMT3B or DNMT1 domain or homologue thereof; (c) an ATR comprising a DNA-binding domain operably linked to a DNMT3L domain or homologue thereof; and (d) an ATR comprising a DNA-binding domain operably linked to a SETDB1 domain or homologue thereof, wherein at least two of the ATRs are selected from different groups (a), (b), (c) or (d).