Cytoplasmic expression system
US12157766B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 14, 2018 |
| Grant date | Dec 3, 2024 |
| Priority date | — |
| Expiry date | Jul 21, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/92
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides host cells having an oxidizing cytoplasm, which are capable of growing to high cell densities and producing recombinant proteins in soluble form. Also provided are methods for growing such host cells to high densities, methods for optionally modulating the growth rates of the host cells, and methods for inducing the host cells to produce the desired gene product in soluble form. Further aspects of the invention relate to host cell preparation and storage methods, which utilize the advantages of producing gene products in host cell cytoplasm to provide methods for long-term storage and/or transport of expressed gene products that are retained in a stable and soluble form within the host cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.