Methods of measuring exosomes using intrinsic fluorescence
US12158424B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 28, 2022 |
| Grant date | Dec 3, 2024 |
| Priority date | — |
| Expiry date | Mar 28, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/76
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Described herein are novel rapid and reliable methods of detection of extracellular vesicles and quantifying extracellular vesicle concentrations and absolute number from various sources, including raw cell harvest. The methods described herein comprise detection of intrinsic fluorescence of extracellular vesicles in biological samples. Extracellular vesicles analyzed by the methods of this application have a stereotypical elution profile distinct from known contaminants. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.