Reagents and methods used in deprotection of 3'-O-amino polynucleotides
US12163172B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 3, 2024 |
| Grant date | Dec 10, 2024 |
| Priority date | — |
| Expiry date | May 3, 2044 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This disclosure relates to an enzymatic method of synthesizing a polynucleotide, comprising a deprotecting step which uses as a deprotecting agent a specific phosphonate compound. It also pertains to a method for deprotecting 3′-O-amino elongated fragments of a polynucleotide in an enzymatic method of synthesizing a polynucleotide, comprising contacting the elongated fragments with this phosphonate compound. This disclosure further relates to a kit for synthesizing a polynucleotide comprising one or more vials of synthesis reagents, at least one of which contains an effective amount of a phosphonate compound and to a specific method for preparing said phosphonate compound.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.