Patent · US Active

In situ sequencing of RNA transcripts with non-uniform 5 prime ends

US12163190B2 · kind B2 · utility

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7References

7Claims

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Assignee

Insitro, Inc. · US

Inventors

Cynthia Hao · Saratoga, US
Max R. Salick · San Francisco, US
Ci Chu · Gongguan, TW

Key dates

Filing date	Jun 16, 2023
Grant date	Dec 10, 2024
Priority date	—
Expiry date	Jun 16, 2043

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6853
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Disclosed herein are methods for performing in situ sequencing of RNA transcripts with non-uniform 5′ ends. During reverse transcription (RT) of RNA transcripts, RT enzyme is induced to “template-switch” to a separate oligonucleotide provided as the template for the upstream flanking region. This flanking region is grafted onto the beginning of the cDNA, enabling padlock probe detection, rolling circle amplification, and fluorescent in situ sequencing. Overall, the disclosed method for in situ sequencing can be applicable for analyzing exogenously introduced transcripts (e.g., identifying and determining impact of a perturbation including a CRISPR perturbation or shRNA/siRNA/ASO perturbation), analyzing naturally occurring transcripts (e.g., measuring gene expression, detecting splicing events), and analyzing modified, naturally occurring transcripts (e.g., detecting mutations or gene edits).

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.