Apparatuses and methods involving protein exploration through proteolysis and nanopore translocation
US12163948B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 21, 2018 |
| Grant date | Dec 10, 2024 |
| Priority date | — |
| Expiry date | Jan 20, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/26
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Embodiments in accordance with the present disclosure are directed to methods and apparatuses used for evaluating a protein in a sample using proteolysis and translocation. An example method includes isolating a single protein from a sample in a chamber having at least a first well and a second well separated from the first well by a membrane with a nanopore, the nanopore providing fluidic communication between the first and second wells. The single protein is cleaved into a plurality of peptide fragments via exposure to a protease in the first well, and translocated through the nanopore by applying an electric potential to the nanopore in the chamber after cleavage of the single protein by the protease. The method further includes detecting events indicative of the translocations of the plurality of peptide fragments through the nanopore and to the second well.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.