Patent · US Active

Nano robotic system for high throughput single cell DNA sequencing

US12163979B2 · kind B2 · utility

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27Claims
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Key dates

Filing dateOct 27, 2022
Grant dateDec 10, 2024
Priority date
Expiry dateFeb 25, 2043

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01Q80/00
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

A nano scale robotic system for single cell DNA sequencing of a strand of DNA positioned on a slide utilizes an atomic force microscope (AFM) having an end effector in the form of a cantilever with a tip. The AFM causes its cantilever tip to scan over the base pairs of the DNA strand. A pair of spaced-apart electrodes at the tip makes contact with opposite sides of the DNA strand and the current between bases of the DNA strand is measured by a current measurement system connected to the electrodes. An artificial intelligence-based data analytic system determines the DNA sequence based on the current from the current measuring system. The AFM tip is guided over the DNA strand by comparing compressed desired intensity local scan images and compressed actual intensity local scan images and using the difference to control the location of the tip.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.