Recombinant KOD polymerase

US12168785B2 · kind B2 · utility

Assignee

• BGI SHENZHEN · CN

Inventors

• Lili Zhai · Beijing, CN
• Lin Wang · University City, US
• Wenwei Zhang · Longbeilingcun, CN
• Yuliang Dong · Longbeilingcun, CN
• Yue Zheng · Singapore, SG
• Fen Liu · 流花街道, CN

Key dates

• Filing date: Sep 3, 2018
• Grant date: Dec 17, 2024
• Expiry date: Mar 17, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/21
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided is a recombinant KOD polymerase, which is the following A) or B): the polymerase shown in A) is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 18 positions in a wild-type KOD DNA polymerase amino acid sequence: 675th, 385th, 710th, 674th, 735th, 736th, 606th, 709th, 347th, 349th, 590th, 676th, 389th, 589th, 680th, 384th, 496th and 383rd; the polymerase described by B) is a protein having DNA polymerase activity that is derived from A) by adding a tag sequence to an end of the amino acid sequence of the protein shown in A).