Native microfluidic CE-MS analysis of antibody charge heterogeneity
US12169203B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 30, 2020 |
| Grant date | Dec 17, 2024 |
| Priority date | — |
| Expiry date | Sep 30, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N1/4044
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for detecting and/or discriminating between post-translational modification variants of an antibody of interest in a sample. The methods including: contacting a sample comprising one or more antibodies of interest with a protease to digest the sample into antibody fragments; separating antibody fragments by molecular weight and/or charge in one or more capillaries using capillary electrophoresis; eluting separated antibody fragments from the one or more capillaries; and determining the mass of the eluted antibody fragments by mass spec analysis, thereby detecting and/or discriminating between post-translational modification variants of the antibody of interest.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.