Method for safely producing botulinum neurotoxin
US12171815B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 18, 2020 |
| Grant date | Dec 24, 2024 |
| Priority date | — |
| Expiry date | Jan 5, 2042 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to a method for producing botulinum toxin in various fragments to then be reassembled, for safely producing same. In the present invention, devised is a method in which: botulinum toxin is produced in fragments by cleaving light and heavy chains thereof into two or three pieces, respectively, and then combined as a full-length toxin, thereby allowing high complexity in production, due to toxicity, as well as low safety and economic feasibility, to be overcome; production of water-soluble botulinum toxin is enabled by using bacteria, thereby markedly shortening the production time as compared to existing production methods; and conjugation of the produced fragments with other proteins and nanoparticles is also enabled, thereby increasing the pharmaceutical extensibility of the toxin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.