Materials and methods for controlling gene editing
US12173290B2 · kind B2 · utility
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Key dates
| Filing date | Jun 26, 2020 |
| Grant date | Dec 24, 2024 |
| Priority date | — |
| Expiry date | Dec 15, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present application provides a CRISPR/Cas system comprising a nuclease segment that encodes a Cas9 nuclease or variant thereof, a guide RNA segment comprising a nucleotide sequence that encodes a gRNA or sgRNA, and a promoter segment comprising a nucleotide sequence that encodes a first promoter comprising one or more tetracycline operator sequence, wherein the gRNA segment is operably linked to the promoter segment. The present application also provides materials and methods for controlling transcriptional expression of guide RNAs and/or post-transcriptional expression of Cas nuclease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.