Organelle genome modification using polynucleotide guided endonuclease
US12173295B2 · kind B2 · utility
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Key dates
| Filing date | Jan 19, 2024 |
| Grant date | Dec 24, 2024 |
| Priority date | — |
| Expiry date | Jan 19, 2044 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07049
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.
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