Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions
US12174176B2 · kind B2 · utility
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9References
5Claims
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Key dates
| Filing date | Dec 16, 2021 |
| Grant date | Dec 24, 2024 |
| Priority date | — |
| Expiry date | Jul 22, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2533/107
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention is directed to kits for identifying the presence of one or more target nucleotide sequences in a sample that involve a ligation and/or polymerase reaction. In some embodiments, the ligation products formed in the ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and the presence of one or more target nucleotide sequences in the sample is identified based on the detection.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.