Systems and methods for electrochemical point-of-care detection of hemoglobin
US12174202B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 5, 2023 |
| Grant date | Dec 24, 2024 |
| Priority date | — |
| Expiry date | Jun 5, 2043 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/726
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method includes lysing the red blood cells of a whole blood sample, oxidizing the free hemoglobin in the lysed sample, and cleaving FVH from the hemoglobin A1C to form an electrochemical test solution. A first portion of the electrochemical test solution is reacted with fructosyl peptide oxidase and a reduced ruthenium mediator to form a first reaction product. A first electrical property of the first reaction product is measured, the measurement being indicative of hemoglobin A1C in the blood sample. A second portion of the electrochemical test solution is reacted with ferrocyanide to form a second reaction product. A second electrical property of the second reaction product is measured, the measurement being indicative of total hemoglobin in the blood sample. Hemoglobin A1C, total hemoglobin, and % HbA1C are determined based on the first and second electrical properties. Also provided are systems and components useful in performing the disclosed methods.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.