Variable region sequence of broad-spectrum antibody against clothianidin and dinotefuran and preparation of intact recombinant antibody thereof

US12180304B2 · kind B2 · utility

Assignee

• ZHEJIANG UNIVERSITY · CN

Inventors

• Yirong Guo · Zhejiang, CN
• Yunyun Chang · Zhejiang, CN
• Ying Zhao · New York, US
• Pengyan Liu · Zhejiang, CN
• Ying Liu · Hangzhou City, CN
• Yang Chen · Ambler, US
• Guonian Zhu · Zhejiang, CN

Key dates

• Filing date: Jul 28, 2021
• Grant date: Dec 31, 2024
• Expiry date: Sep 8, 2043

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/92
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides a variable region sequence of a broad-spectrum antibody against clothianidin and dinotefuran, where a gene encoding a heavy chain variable region has an amino acid sequence shown in SEQ ID NO: 2. The present disclosure further discloses a broad-spectrum intact recombinant antibody against clothianidin and dinotefuran, including a heavy chain constant region, a heavy chain variable region, a light chain constant region, and a light chain variable region, where a gene encoding the heavy chain variable region has an amino acid sequence shown in SEQ ID NO: 2. The sequence genes obtained by the present disclosure are ligated to an expression vector containing a heavy chain constant region gene and a light chain constant region gene, respectively, and an intact recombinant antibody is expressed and obtained by using mammalian cells with a double-plasmid system.