Patent · US Active

Methods for reprograming non-pluripotent cells into pluripotent stem cells

US12180509B2 · kind B2 · utility

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20Claims
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Key dates

Filing dateJul 22, 2021
Grant dateDec 31, 2024
Priority date
Expiry dateDec 17, 2041

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2506/23
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

Provided are chemical inducers of pluripotency (CIP) which include glycogen synthase kinase inhibitors, TGFβ receptor inhibitors, cyclic AMP agonists and S-adenosylhomocysteine hydrolase (SAH) inhibitors or histone acetylators. A method of inducing pluripotency in a partially or completely differentiated cell by using such chemical inducers of pluripotency is also provided. The method includes: (i) contacting a cell with the CIPs for a sufficient period of time to result in reprograming the cell into a pluripotent stem cell having ESC-like characteristics (CiPSC). Isolated chemically induced pluripotent stem cells (CiPSCs) and their progeny, produced by inducing differentiation of the CiPSCs, can be used in a number of applications, including but not limited to cell therapy and tissue engineering.

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