Lipid-preserving refractive index matching for prolonged imaging depth for transparent tissue sample and composition

US12181386B2 · kind B2 · utility

Assignees

• CASE WESTERN RESERVE UNIVERSITY · US
• UNIVERSITY HOSPITALS CLEVELAND MEDICAL CENTER · US
• VANDERBILT UNIVERSITY · US

Inventors

• Michael Jenkins · Cleveland, US
• Yehe Liu · Cleveland, US

Key dates

• Filing date: Nov 9, 2018
• Grant date: Dec 31, 2024
• Expiry date: Feb 28, 2042

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/5082
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present disclosure relates to an optical clearing method called lipid-preserving index matching for prolonged imaging depth (LIMPID). The optical clearing method can include simply immersing a sample in an optical clearing solution before imaging (e.g., with microscopy or optical coherence tomography). The optical clearing solution can include water; an iodine-containing non-ionic radiocontrast agent; and urea.