Method and probe for monitoring oxygen status in live mammalian cells

US12181414B2 · kind B2 · utility

Assignee

• UNIVERSITY COLLEGE CORK—NATIONAL UNIVERSITY OF IRELAND, Cork · IE

Inventors

• Dmitri Boris Papkovsky · Moskovskiy, RU
• Andreas Fercher · Cork, IE
• Ingo Klimant · Labuch, AT
• Sergey Olegovich Borisov · Novosibirsk, RU
• Alexander Zhdanov · Cork, IE

Key dates

• Filing date: Dec 5, 2019
• Grant date: Dec 31, 2024
• Expiry date: Apr 20, 2041

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/84
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method of determining oxygen concentration, metabolic activity, and/or the effects of a test substance on the metabolic activity of a live cell sample by photoluminescence quenching technique employing a photoluminescent probe that self-loads sans any loading reagent into the cells of the cell sample. The probe comprises a plurality of polymeric particles each comprising an amphiphilic cationic polymer matrix having a hydrophobic core and a hydrophilic positively charged surface provided by quaternary amino groups, and a hydrophobic oxygen-sensitive photoluminescent dye embedded in the hydrophobic core.