Method for efficiently collecting and purifying Orobanche cumana (O. cumana) germination stimulants using aeroponic system and solid-phase extraction (SPE)
US12185726B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Jun 5, 2020 |
| Grant date | Jan 7, 2025 |
| Priority date | — |
| Expiry date | Apr 3, 2043 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P60/21
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The patent discloses a method for efficiently collecting and purifying Orobanche cumana (O. cumana) germination stimulants using aeroponic system and solid-phase extraction (SPE), including the following steps: (1) sunflower seeds germination, then planting sunflower seedlings in aeroponic device, and cultivating the sunflower seedlings in the aeroponic system; at the aeroponic stage, phosphorus-containing aeroponic nutrient solution is first used to cultivate the sunflower seedlings for 20 to 25 days, and phosphorus-free aeroponic nutrient solution is then used instead to subject the sunflower seedlings to starvation cultivation for 5 to 7 days; and (2) passing all nutrient solutions in the aeroponic device through an SPE cartridge for SPE to extract O. cumana germination stimulants. The obtained O. cumana germination stimulants are diversified, and have high concentration and purity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.