Passive mixing microfluidic urinary albumin chip (UAL-chip) for chronic kidney disease
US12186753B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 24, 2019 |
| Grant date | Jan 7, 2025 |
| Priority date | — |
| Expiry date | Dec 16, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/347
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Described herein is a method for mixing unequal amounts of two reagents to produce a detectable reaction in a microfluidic chip. In one example, there is a fluorescent microfluidic urinary albumin chip (UAL-Chip) that exploits the nonimmunological fluorescent assay. In this chip, we constructed a passive and continuous mixing module, in which the loading process requires only an inexpensive dropper, and the signal is stable over time, as discussed below. We applied a pressure-balancing strategy based on the immiscible oil coverage which highly improves the precision in controlling the mixing ratio of sample and dye. The UAL-Chip has achieved an estimated limit of detection (LOD) of 8.4 μg/ml using albumin standards, which is below the 30 μg albumin per ml urine level considered to be indicative of kidney damage.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.