Co-receptor affinity enhanced major histocompatibility class II molecules

US12187781B2 · kind B2 · utility

Assignee

• Regents of the University of Minnesota · US

Inventors

• Marc K. Jenkins · Minneapolis, US
• Thamotharampillai Dileepan · Minneapolis, US
• Deepali Malhotra · Minneapolis, US

Key dates

• Filing date: Aug 1, 2019
• Grant date: Jan 7, 2025
• Expiry date: Apr 12, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present disclosure provides variant major histocompatibility complex class II (MHCII) beta chains, as well as heterodimers and multimers including MHCII alpha chains and the variant MHCII beta chains. Also provided by the present disclosure are nucleic acids, expression cassettes, and expression vectors encoding the MHCII beta chains. The heterodimers and multimers of the present disclosure have a higher affinity for CD4 co-receptors than comparable reagents including wild type MHCII beta chains, and therefore are advantageous for use in methods of phenotyping or activating CD4+ T cells.