Products and processes for nucleic acid detection and quantification
US12188083B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | May 31, 2019 |
| Grant date | Jan 7, 2025 |
| Priority date | — |
| Expiry date | Aug 27, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are products and processes for primer extension reactions using chain terminating reagents and enzymes having 3′ to 5′ exonuclease activity, whereby unextended oligonucleotides are digested and extended oligonucleotides are not digested. Also provided herein are products and processes for the detection of the presence, absence or amount of one or more variants of a target nucleic acid species or a plurality of target nucleic acid species comprising primer extension reactions using chain terminating reagents and enzymes having 3′ to 5′ exonuclease activity, whereby unextended oligonucleotides are digested and extended oligonucleotides are not digested.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.