Method for highly sensitive DNA methylation analysis
US12188084B2 · kind B2 · utility
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19Claims
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Key dates
| Filing date | Sep 27, 2021 |
| Grant date | Jan 7, 2025 |
| Priority date | — |
| Expiry date | Sep 9, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods, compositions and kits are provided to amplify an amount of genomic methylated DNA that can be subsequently analyzed and/or sequenced. It has particular use with small amounts of DNA, including, but not limited to cell free DNA samples. In some embodiments, the ratio of polymerase and methyltransferase is controlled in order to provide maximum yields. In some embodiments, a dual primase/polymerase is used.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.