Hormone-free culture method for citrus shoot tip
US12193380B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 6, 2023 |
| Grant date | Jan 14, 2025 |
| Priority date | — |
| Expiry date | Aug 24, 2043 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P60/21
- WIPO fieldFood chemistry
- WIPO sectorChemistry
Abstract
A hormone-free culture method for a citrus shoot tip is provided according to the following steps: (1) selecting a citrus branch to make an explant; (2) washing with sterile water after disinfection; (3) inserting the explant into a Murashige and Skoog (MS) solid medium and culturing to produce a branch with new buds; (4) preparing a Murashge and Tucker (MT) liquid medium solution, where a mass concentration of sucrose is 7-8%; (5) removing an outer leave of the new bud, cutting and taking a shoot tip, and inoculating in a liquid medium for primary culture; (6) selecting a seed of a trifoliate orange or a citrange and peeling off a testa; washing with the sterile water after disinfection; (7) inoculating a resulting seed into a test tube containing the MS solid medium and culturing in dark to obtain an etiolated seedling; (8) cutting a T-shaped incision and placing the shoot tip in a middle of the T-shaped incision to obtain a grafted seedling; and (9) grafting culture, and then conducting a secondary grafting in a greenhouse when a scion bud grows to 0.5-1 cm. According to the present invention, regenerated seedlings are obtained without using hormones, and the efficiency of plant…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.