SiRNA delivery system compound as well as preparation method and application thereof
US12194145B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Apr 12, 2021 |
| Grant date | Jan 14, 2025 |
| Priority date | — |
| Expiry date | Nov 16, 2043 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K31/713
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention relates to the technical field of gene treatment and nano targeted delivery, in particular to a siRNA delivery system compound as well as a preparation method and application thereof. The preparation method comprises the following steps of reacting Boc-protected histidine with cholesterol to generate Boc-protected histidine cholesteryl ester, and removing BOC-released protected amino in trifluoroacetic acid to generate histidine cholesteryl ester; then, enabling amino to react with carboxyl of carboxymethyl chitosan to modify long-chain carboxymethyl chitosan to generate carboxymethyl chitosan modified by histidine cholesteryl ester, and performing targeted labeling on carboxymethyl chitosan modified by histidine cholesteryl ester to generate a carboxymethyl chitosan high-molecular compound modified by histidine cholesteryl ester and a targeted marker together, then forming a stable spherical delivery vector through self-assembly above critical micelle concentration, and mixing the stable spherical delivery vector with siRNA (small interfering ribonucleic acid) to obtain the siRNA delivery system compound.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.