Reversible protein multimers, methods for their production and use
US12195556B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 14, 2018 |
| Grant date | Jan 14, 2025 |
| Priority date | — |
| Expiry date | Nov 28, 2041 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K2039/64
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Some aspect of this disclosure provide reversible MHC protein multimers, and methods of using such multimers in the detection and/or isolation of specific T-cells or T-cell populations. Because reversible MHC multimers can efficiently be dissociated, the time of MHC binding to T-cell receptors, and, thus, T-cell receptor-mediated T-cell activation can be minimized. The use of reversible MHC multimers as provided herein, accordingly, allows for the detection and isolation of bona fide antigen-specific CD8+ T cells without inducing activation dependent cell death, including rare, therapeutically valuable T-cells expressing T-cell receptors binding tumor antigens with high affinity. Methods for the production and use of reversible multimers are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.