Methods of analyzing capped ribonucleic acids
US12195794B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 22, 2019 |
| Grant date | Jan 14, 2025 |
| Priority date | — |
| Expiry date | Mar 21, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5308
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided are methods of analyzing capped ribonucleic acids (RNAs). The methods include translocating an adapted RNA through a nanopore of a nanopore device. The adapted RNA includes an RNA region, a 5′ cap, and an adapter polynucleotide attached to the 5′ cap. The methods include monitoring ionic current through the nanopore during the translocating, translocating the 5′ cap through the nanopore, and identifying one or more ionic current features characteristic of the 5′ cap (e.g., a triphosphate linkage between the 5′ cap and nucleotide N1 of the RNA region, a 5′ to 5′ orientation of the 5′ cap and nucleotide N1 of the RNA region, and/or the like), translocating through the nanopore. Also provided are computer-readable media, computer devices, and systems that find use, e.g., in practicing the methods of the present disclosure.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.