Automated nested recombinase polymerase amplification
US12195813B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 3, 2017 |
| Grant date | Jan 14, 2025 |
| Priority date | — |
| Expiry date | Mar 3, 2037 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/0887
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A flu assay system including a sample module, a microfluidic nucleic acid amplification device, and an analyzer to facilitate fully automated nested recombinase polymerase amplification (RPA) on a sample delivered to the nucleic acid amplification device via the sample module. The assay includes providing a sample to a microfluidic device, and amplifying a target polynucleotide sequence in the sample. Amplifying the target polynucleotide sequence includes performing a first round of amplification on the sample to yield a first amplification product, and performing a second round of amplification on the first amplification product to yield a second amplification product. The second amplification product includes a smaller sequence completely contained within the first amplification product produced during the first round of amplification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.