Method for analyzing glycosaminoglycan
US12196743B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Oct 14, 2021 |
| Grant date | Jan 14, 2025 |
| Priority date | — |
| Expiry date | Oct 14, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/04
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided is a method by which the disaccharides derived from glycosaminoglycans can be analyzed in a stable and highly reproducible manner. A method for analyzing a glycosaminoglycan according to the present invention includes: a first process for producing a plurality of kinds of disaccharides derived from a glycosaminoglycan in a biological sample by adding a plurality of kinds of glycosaminoglycan-specific enzymes to the biological sample; and a second process for separating and analyzing the plurality of kinds of disaccharides by a liquid chromatography-mass spectrometry method, where a column used for liquid chromatography in the liquid chromatography-mass spectrometry method is a column packed with a stationary-phase support to which an amide group as a functional group is bound, or a column packed with a stationary-phase support to which an adamantyl group as a functional group is bound.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.