Methods for heat-assisted enzyme digestion
US12202857B2 · kind B2 · utility
0Cited by
10References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 7, 2021 |
| Grant date | Jan 21, 2025 |
| Priority date | — |
| Expiry date | Jun 1, 2043 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/976
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present disclosure relates to a method of digesting a sample comprising protein, the method comprising: adding the sample to an apparatus containing a buffer and a solid support surface comprising a surface coating, wherein the surface coating immobilizes enzyme while reducing undesired interactions between the sample and the solid support surface; immobilizing enzymes on the surface coating for digestion of the protein of the sample; and heating the sample to complete a heat-assisted digestion of the protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.