Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
US12209266B2 · kind B2 · utility
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Key dates
| Filing date | May 11, 2021 |
| Grant date | Jan 28, 2025 |
| Priority date | — |
| Expiry date | Jun 3, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2521/131
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein include methods of generating a single stranded deoxyribonucleic acid (ssDNA) scaffold comprising nucleotides with modified bases using a recombinant terminal deoxynucleotidyl transferase (TdT). The recombinant TdT can comprise an amino acid sequence that is at least 80% identical to a Bos taurus TdT, or a fragment thereof, and for example comprise one or more amino acid substitution mutations at one or more positions functionally equivalent to Glu191, Lys193, Glu194, Asp242, Lys287, Phe296, Met299, Thr342, and His420 in the Bos taurus TdT.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.