Methods of forming multi-color fluorescence-based flow cytometry panel
US12209948B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 26, 2021 |
| Grant date | Jan 28, 2025 |
| Priority date | — |
| Expiry date | Jun 26, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/1493
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.