Three-dimensional fluorescence imaging system comprising single-objective light-sheet microscopy
US12210144B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 12, 2024 |
| Grant date | Jan 28, 2025 |
| Priority date | — |
| Expiry date | May 12, 2044 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/105
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A three-dimensional fluorescence imaging system, including an illumination objective, a scanning lens group, a galvanometer, an illumination module, and a detection module. The scanning lens group includes a first scanning lens and a second scanning lens; main optical axes of the first scanning lens and the second scanning lens are orthogonal and confocal; a common focus of the first scanning lens and the second scanning lens is disposed on a central part of the galvanometer. The illumination module is configured to generate an illumination laser beam; the illumination laser beam passes through the scanning lens group and enters a rear pupil plane of the illumination objective, forming a light sheet having an inclination angle α projected onto an excitation plane of a sample to excite fluorescence; the excitation plane is diagonally intersected with a main optical axis of the illumination objective. The detection module includes an area array detector.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.