Method for identifying base in nucleic acid and system
US12211589B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 10, 2020 |
| Grant date | Jan 28, 2025 |
| Priority date | — |
| Expiry date | Oct 11, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/20221
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
A method for identifying a base in nucleic acid, a computer-readable storage medium, a computer program product, and a system. The method for identifying a base in nucleic acid comprises: mapping a coordinate of each bright spot in a bright spot set corresponding to a template onto an image to be inspected, and determining the position of a corresponding coordinate on said image (S11); determining the intensity of a signal at the position of the corresponding coordinate on said image, the intensity being a corrected intensity (S21); and comparing the intensity of the signal at the position of the corresponding coordinate on said image with the size of a first preset value, and determining a base type corresponding to the position on the basis of the comparison result, so as to achieve base calling (S31). The method may quickly and accurately identify a base, and achieve the determination of an order of nucleotides/bases of at least part of a sequence of a template.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.