Linker structures with minimal scar for enzymatic synthesis
US12215121B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 20, 2023 |
| Grant date | Feb 4, 2025 |
| Priority date | — |
| Expiry date | Jan 20, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC25B3/20
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This disclosure provides electrochemically-cleavable linkers with cleavage potentials that are less than the redox potential of the solvent in which the linkers are used. In some applications, the solvent may be water or an aqueous buffer solution. The linkers may be used to link a nucleotide to a bound group. The linkers include a cleavable group which may be one of a methoxybenzyl alcohol, an ester, a propargyl thioether, or a trichloroethyl ether. The linkers may be cleaved in solvent by generating an electrode potential that is less than the redox potential of the solvent. In some implementations, an electrode array may be used to generate localized electrode potentials which selectively cleave linkers bound to the activated electrode. Uses for the linkers include attachment of blocking groups to nucleotides in enzymatic oligonucleotide synthesis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.