Methods of targeted genetic alteration in plant cells
US12215329B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 15, 2018 |
| Grant date | Feb 4, 2025 |
| Priority date | — |
| Expiry date | Feb 15, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The current invention relates to methods of targeted genetic alteration in cells, preferably plant cells, as well as to plant cells and plants thus obtained using at least a fusion protein comprising a site-specific nuclease domain and a deaminase domain, or a construct encoding the same. The method also provides for a composition and a kit comprising a combination of a first fusion protein comprising a cytosine deaminase domain and a second fusion protein comprising an adenine deaminase domain, preferably for use in the method of the invention. The method provides for targeted alteration of a DNA duplex in plant cells with increased efficacy.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.