Compositions and methods for making epigenetic modifications

US12215361B2 · kind B2 · utility

Assignee

• Whitehead Institute For Biomedical Research · US

Inventors

• Edwin Neumann · Somerville, US
• Tessa Bertozzi · Cambridge, US
• Jonathan Weissman · San Francisco, US

Key dates

• Filing date: Feb 16, 2024
• Grant date: Feb 4, 2025
• Expiry date: Feb 16, 2044

Classification

• Technology area (CPC —): General

Abstract

The disclosure provides, in various embodiments, fusion proteins comprising a DNA-binding domain, a DNMT3A-binding domain, and a H3K4me0; and polynucleotides and vectors encoding one or more of the fusion proteins. The disclosure also provides, in various embodiments, gene-delivery systems, cells, compositions (e.g., pharmaceutical compositions) and kits comprising one or more of the fusion proteins polynucleotides, or vectors; methods of epigenetically modifying a genomic locus in a cell; and methods of treating a subject (e.g., a human) in need thereof.