Balanced indels
US12215363B2 · kind B2 · utility
0Cited by
1References
12Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Mar 6, 2020 |
| Grant date | Feb 4, 2025 |
| Priority date | — |
| Expiry date | Apr 11, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention pertains to the targeted alteration of a duplex DNA in a cell, whereby two site-specific nucleases generate an indel, such that the open reading frame is not altered after the second indel. The invention further pertains to the use of such nucleases for the targeted alteration of an open reading frame in duplex DNA and a kit of parts for use in a method of the invention. Using the method of the invention, novel plants were obtained having an improved herbicide resistance. The invention therefore also concerns plants having improved herbicide resistance due to the expression of an altered ALS protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.