Mitochondrial nucleic acid depletion and detection
US12215376B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jun 20, 2019 |
| Grant date | Feb 4, 2025 |
| Priority date | — |
| Expiry date | Mar 18, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/70
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a method for depleting host nucleic acid in a biological sample, said sample having been previously obtained from an animal host and having been subjected to treatment to lyse host cells present in the sample, and having been subjected to a treatment to deplete nucleic acid released from host cells within said sample or otherwise to render such nucleic acid unidentifiable, the method comprising: (a) depleting host mitochondria or host mitochondrial DNA (mtDNA) present in the treated sample; and (b) extracting and/or analysing remaining nucleic acid from the treated sample. Also provided is a related kit comprising reagents for performing the method. Further claimed is a method for isolating mitochondrial DNA after host cells have been lysed and host cell nucleic acids have been removed from the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.