Patent · US Active

Automatic calibration using machine learning

US12223754B2 · kind B2 · utility

0Cited by
1References
13Claims
0Family size

Assignee

Inventor

Key dates

Filing dateJun 7, 2022
Grant dateFeb 11, 2025
Priority date
Expiry dateJun 9, 2043

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG06T2207/20084
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

There is provided a cell analysis apparatus that comprises image capture circuitry for capturing a brightfield image of a cell using brightfield imaging. The cell has been dyed by a functional dye that indicates, during fluorescence imaging and during brightfield imaging, whether the cell has a given characteristic. A model derived by machine learning is stored and used in combination with the brightfield image to determine whether the cell has the given characteristic. There is also provided a method for creating a cell categorisation model, comprising applying a functional dye to one or more samples comprising a plurality of cells. The functional dye indicates during fluorescence imaging and during brightfield imaging whether each of the cells has a given characteristic. A brightfield image and a corresponding fluorescence image for each of the plurality of cells to which the dye has been applied are captured and a machine learning process is used to generate a model that predicts whether a cell has the given characteristic from a brightfield image. The model is generated by using the brightfield image and the corresponding fluorescence image of each of the plurality of cells as …

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