Methods and compositions for manipulating nucleic acids
US12226745B2 · kind B2 · utility
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21References
18Claims
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Key dates
| Filing date | May 3, 2019 |
| Grant date | Feb 18, 2025 |
| Priority date | — |
| Expiry date | Aug 24, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2563/149
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.