Methods and systems for high-throughput cell processing
US12227729B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 24, 2021 |
| Grant date | Feb 18, 2025 |
| Priority date | — |
| Expiry date | Sep 5, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M39/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Described herein are methods and systems for cell processing or, more specifically, for introducing various payloads into cells. These methods and systems use a mechanoporation approach in which cells are rapidly compressed and then released to relax while absorbing the payload. More specifically, these methods and systems enable high-throughput mechanoporation with various clogging mitigation features. A cell processing apparatus comprises a shell with an inner shell cylindrical surface, a core with an outer core cylindrical surface, and ridges, supported on and protruding away from one of the inner shell cylindrical surface and the outer core cylindrical surface. The core is disposed inside the shell. The outer core cylindrical surface is concentric with the inner shell cylindrical surface. Each of the ridges forms a ridge gap with the other one of the inner shell cylindrical surface and the outer core cylindrical surface.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.