Methods and compositions for treating negative-sense single-stranded RNA virus

US12227769B2 · kind B2 · utility

Assignees

• THE UNIVERSITY OF CHICAGO · US
• NATIONWIDE CHILDREN'S HOSPITAL, INC. · US
• OHIO STATE UNIVERSITY · US

Inventors

• Jianrong Li · Dublin, US
• Mark E. Peeples · Bexley, US
• Chuan He · Chicago, US
• Stefan NIEWIESK · Dublin, US
• Mijia Lu · Columbus, US
• Miaoge Xue · Columbus, US
• Zijie Zhang · Chicago, US
• Boxuan ZHAO · Chicago, US

Key dates

• Filing date: Oct 18, 2019
• Grant date: Feb 18, 2025
• Expiry date: Jun 2, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2760/18562
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The current disclosure relates to methods, compositions and kits for detecting modified adenosine in a target RNA molecule. Aspects relate to a method for detecting modified adenosine in a target ribonucleic acid (RNA) comprising contacting the target RNA with an adenosine deaminase enzyme (adenosine deaminase, RNA-specific) to generate a target RNA with deaminated adenosines and sequencing the target RNA with deaminated adenosines; wherein the modified adenosine is detected when the nucleotide sequence includes adenosine within a m6A motif.