Patent · US Active

Multi-stage sample recovery system

US12228584B2 · kind B2 · utility

0Cited by
95References
24Claims
0Family size

Assignee

Inventor

Key dates

Filing dateOct 20, 2021
Grant dateFeb 18, 2025
Priority date
Expiry dateOct 2, 2043

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N2035/1053
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Multi-stage sample-recovery systems, including automated 2-stage and 3-stage sample-recovery systems, are provided. Such systems enable the rapid screening and recovery of samples, including viable cell-based samples, from high-throughput screening systems, including systems utilizing large-scale arrays of microcapillaries. In specific screening systems, each microcapillary comprises a solution containing a variant protein, an immobilized target molecule, and a reporter element. Immobilized target molecules may include any molecule of interest, including proteins, nucleic acids, carbohydrates, and other biomolecules. The association of a variant protein with a molecular target is assessed by measuring a signal from the reporter element. The contents of microcapillaries identified in the assays as containing variant proteins of interest can be identified and recovered using the multi-stage systems disclosed herein.

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