Patent · US Active

Point-of-care-computational microscopy based-systems and methods

US12228723B2 · kind B2 · utility

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20Claims
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Key dates

Filing dateFeb 6, 2024
Grant dateFeb 18, 2025
Priority date
Expiry dateFeb 6, 2044

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG06T2207/30024
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Described herein are systems and methods for assessing a biological sample. The methods include: characterizing a speckled pattern to be applied by a diffuser; positioning a biological sample relative to at least one coherent light source such that at least one coherent light source illuminates the biological sample; diffusing light produced by the at least one coherent light source; capturing a plurality of illuminated images with the embedded speckle pattern of the biological sample based on the diffused light; iteratively reconstructing the plurality of speckled illuminated images of the biological sample to recover an image stack of reconstructed images; stitching together each image in the image stack to create a whole slide image, wherein each image of the image stack at least partially overlaps with a neighboring image; and identifying one or more features of the biological sample. The methods may be performed by a near-field Fourier Ptychographic system.

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